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Promises of organoids for livestock research

Mis à jour le 03/05/2018
Publié le 20/04/2018
Mots-clés : CONFÉRENCES

Vendredi 15 juin à 13h30 (durée 45 min)

Centre INRA IdF Jouy-en-Josas

Bâtiment 231, salle 271.

Abstract

Organoids are 3D organotypic cultures derived from tissue stem cells, embryonic cells and induced pluripotent stem cells (iPSCs), that mimic the structure and function of organs ranging from the colon1 to the brain2. They are amenable to essentially all cell biological and molecular technologies that are commonly used for cell lines, including genome editing with CRISPR-Cas technology3. Organoids are proving to be an enabling tool for applications ranging from fundamental biology to personalized or regenerative medicine (Method of Year 2017, Nat. Methods). Organoids grown from adult stem cells (obtained from tissue biopsies of almost any organ and animal species) provide a potentially powerful research platform e.g. for ex vivo phenotyping in livestock breeding programs4 and investigating genotype-to-phenotype relationships. Organoids derived from pluripotent stem cells are increasingly used in human biology and for designing new therapies; they provide controlled genetic backgrounds and the ability to drive their differentiation into virtually any cell type.

This talk will provide an overview of organoids and their employment in fundamental and applied research, with examples emerging in livestock.

 

  1. Date, S. & Sato, T. Mini-gut organoids: reconstitution of the stem cell niche.Annu Rev Cell Dev Biol31, 269-289, doi:10.1146/annurev-cellbio-100814-125218 (2015).
  2. Di Lullo, E. & Kriegstein, A. R. The use of brain organoids to investigate neural development and disease.Nat Rev Neurosci18, 573-584, doi:10.1038/nrn.2017.107 (2017).
  3. Driehuis, E. & Clevers, H. CRISPR/Cas 9 genome editing and its applications in organoids.Am J Physiol Gastrointest Liver Physiol312, G257-G265, doi:10.1152/ajpgi.00410.2016 (2017).
  4. Van der Hee, B.et al.Optimized procedures for generating an enhanced, near physiological 2D culture system from porcine intestinal organoids.Stem Cell Res28, 165-171, doi:10.1016/j.scr.2018.02.013 (2018).