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Development of biosensors for medical and environmental applications

 © R. S. Marks
Mis à jour le 11/07/2016
Publié le 27/06/2016
 © R. S. Marks
© R. S. Marks

Prof. Robert S. Marks

Department of Biotechnology Engineering
The Ben Gurion University of the Negev, Beer-Sheva, Israel

Jeudi 7 juillet 2016 à 11h00
Centre de Jouy-en-Josas
Salle de réunion 800; bâtiment 440


We describe herein the development of various technologies developed in our laboratory over the years, which are mostly multi-disciplinary in scope (chemistry, interfacial chemistry, immunology, molecular biology, physics, data mining and chemical engineering). Most have been validated using real-life samples from various continents. The biosensors are artificially divided in two main categories, affinity (DNA, antibodies) or based on live bioreceptors such as bioreporters. Various transducers are discussed (optical, electrochemical), adapted for point-of-care, on-site testing, laboratory-based or on-line sensing. These include chemiluminescence-based fiber-optic immunosensors to viral pathogens  (Ebola, Dengue, Hepaptitis C, West Nile virus, Rift Valley fever, CCHF) – the BioPen concept; biochip immunosensors based on electrogenerated films deposited on ITO chip surfaces; magnetic liquid fiber guide immunosensors; diagnostic phagocytic chemiluminescent imprints to identify clinical ailments  or other techniques still in development such as the detection of negative stranded RNA viruses (influenza) using reverse genetics cell sensors. Work on nanostructures for enhanced immunosensors or metal enhanced bioluminescence will also be described. We have several techs in the pipeline including lateral flow immunoassay modified with an electrochemical device  to produce a quantitative assay which have been spun off as a company called www.biosensorix.com, or stilbene-aptamer complex-based assays, as well as bioluminescent fiber-optic bioreporter biosensors for water toxicity monitoring. Our latest technology based on a stack immunoassay  is being considered for spinning off…
  Algaar, F.; Eltzov, E.; Vdovenko, M.; Sakharov, I.; Fajs, L.; Weidmann, M.; Mirazimi, A.; Marks, R. (2015) Fiber-optic immunosensor for detection of Crimean-Congo Hemorrhagic fever IgG antibodies in patients. Analytical Chemistry. 10.1021/acs/analchem.5b01728.
  Prilutsky, D., E. Shneider, A. Shefer, B. Rogachev, L. Lobel, M. Last and R. S. Marks (2011) Differentiation between viral and bacterial acute infections using chemiluminescent signatures of circulating phagocytes. Analytical Chemistry. 83: 4258-4265.
  Sinawang, P.D., V. Rai, R.E. Ionescu and R.S. Marks (2016) Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein. Biosensors & Bioelectronics. 77: 400-408.
  Eltzov, E. and R. S. Marks (2016) Miniaturized flow stacked immunoassay detects E. coli in a single step. Analytical Chemistry. Accepted.

Invité par Jasmina Vidic, UR892 Virologie et Immunologie Moléculaires