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Developing innovative cellular systems in pig to study genome organization and function

Un milliard de séquences sur une petite plaque… © BERTRAND Nicolas
Mis à jour le 19/01/2018
Publié le 09/11/2017
SAPS conference FAANG logo. © Inra
© Inra

Dr Hervé Acloque

INRA, Laboratoire GenPhySE UMR1388, Castanet Tolosan

Lundi 20 novembre à 11 h

INRA Centre IdF Jouy-en-Josas

Amphithéâtre du bâtiment 440


The projected increase in world meat demand (some forecasts up to a doubling in 50 years), in connection with population growth and changes in eating habits, require us to rethink our production systems. It is thus necessary to reduce the pressure of livestock production on ecosystems, to improve food and health security and to take account of the welfare of animals.

To achieve these objectives, a better knowledge of the link between genotype and phenotype is necessary and will be possible only by a better knowledge of the biological mechanisms and genome regulatory elements underlying these traits. A strong research effort is now devoted to improve the functional annotation of livestock genomes and to identify genetic variants located in regulatory elements and associated with these complex traits.
Complementary to genome-wide assays, development of efficient cellular tools are also key to answer these biological questions because they allow to reduce complexity, they are easy to manipulate for functional analysis and they are more suitable than living farm animals to generate biological data through high-throughput screens (HTS).

To address these questions we developed in parallel two complementary projects:
(1) the Fr-AgENCODE project aims to improve the functional annotation of the genome of 4 livestock species (chick, pig, cattle, goat) and to identify regulatory elements with differential activity in 3 different cell types (liver cells, CD4+ and CD8+ T-cells).
(2) the UNIPLURI project aims to better characterize and produce pig pluripotent stem cells. These cells can differentiate in vitro and in vivo toward all the cell lineages including the germline and unlike primary cells, PSCs do not enter senescence after long-term culture. As such, these cells can be a powerful cellular platform to evaluate the causal link between genetic polymorphisms and cell phenotypes
I will present on-going results on both project and future research directions that we want to develop, as part of the FAANG international consortium, and in collaboration with national and international collaborators.

Invité par Claire Rogel-Gaillard, UMR 1313 GABI