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Listening to your inner goddess: a spontaneous SCID pig as an emerging model for cancer and regenerative medicine research.

. © Inra, C.K. Tuggle
Mis à jour le 16/07/2019
Publié le 16/06/2019
Mots-clés : CONFÉRENCES

Mercredi 3 juillet 2019 à 10h00

INRA IdF-Jouy-en-Josas

Amphithéâtre du bâtiment 440

Résumé:

Severe Combined Immune Deficient (SCID) mice have been used for many years in xenograft research because the lack of an adaptive immune system permits the growth and subsequent study of human cells in an in vivo environment. However, rodents are an imperfect model for several aspects of human biology and applied medical research. On the other hand, the domestic pig has anatomical, physiological, and genomic similarities to humans that provide significant value as an alternative to rodent models. Further, SCID pigs have been reported, including the spontaneous SCID pig at Iowa State university (ISU). I will describe the serendipitous discovery and characterization of the ISU SCID pig, which has been shown to contain mutations in the Artemis gene, named for the Greek goddess of the hunt, childbirth, and the protector of small children. Artemis is required for normal DNA repair, including somatic recombination necessary for B and T lymphocyte production, and thus Artemis mutations cause this severe immunodeficiency. I will also discuss our new research studies and husbandry protocols for maintaining these highly disease susceptible animals in strict biocontainment. These methods have successfully maintained specific pathogen free SCID pigs for up to 6 months (>100 kg). The ISU SCID pig has been successfully used to study both zoonotic pathogens as well as those that cannot be propagated in rodents, grow several types of human cancers, and study engraftment of human skin. These studies and accompanying animal protocols have established SCID pigs as a valuable large animal xenograft model.

  • Invité par Elisabetta Giuffra, UMR131 Génétique Animale et Biologie Intégrative