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UMS TEFOR Paris-Saclay joins SAPS

 © UMS TEFOR Paris-Saclay
Updated on 09/10/2019
Published on 05/24/2019
Keywords: BREVES

The zebra fish : an interesting model for agronomic research

The zebra fish has emerged as an important vertebrate model for both applied and fundamental research. Its growing popularity among scientists comes from several factors: ease of maintenance of the farms, its rapid development, or its transparency, which is natural for the embryo and later induced by treatment. In addition,its small size makes it very attractive: the adult measures approximately four centimeters, allowing high resolution imaging of an organ or the whole fish in a short time. It has many uses in agronomy: comparative or targeted research of important polymorphisms in breeding fish, clarification of the underlying mechanisms of the main physiological functions, studies associated with farm animal pathologies.

Genome editing and phentoyping by imaging

The TEFOR Paris-Saclay service unit (UMS TPS), a core facility at the heart of the TEFOR infrastructure, draws on its expertise in important biotechnologies available in zebrafish for the implementation of research projects for third-party users. The edition of genomes using CRISP-R/Cas 9 can reproduce interesting mutations. 3D imaging, combined with obtaining hundreds of individuals per generation makes it possible, for example, to robustly evaluate the efficacy of a molecule and its possible teratogenic effects at the level of an entire organism after applying simple balneation.

Closer to the needs of the scientific community

In order to offer complete and innovative services, UMS TPS coordinates a network of center experts: a consortium of partner laboratories and core facilities whose complimentary activities makes it possible to better cover the needs of the scientific community. Project management for third party users is offered through a one-step system: the user has a single contact, ensuring a simplified monitoring of his/her project, with as few administrative procedures as possible.

. © INRA, UMS TEFOR Paris-Saclay
© INRA, UMS TEFOR Paris-Saclay

Technological research to ensure a quality of services

UMS TEFOR Paris-Saclay uses strong multidisciplinary technological research in order to offer continual cutting-edge innovations. For genome editing, they work with the TACGENE core facility (U1154–UMR7196 CNRS/INSERM/MNHN) on "base editing": targeted and fine editing of the genome using deaminases that introduce mutations on one single base. For phenotyping, they lead programs on the opitimization of tools and microscopic methodologies, accompanied with the development of 3D image collecting and optimal navigation tools in these databases.

Towards an optimization of model fish farming

UMS TEFOR Paris-Saclay works hand in hand with different INRA units specialized in the use of fish models (zebrafish, medaka, trout,etc.) in order to improve our understanding of fish breeding techniques for these aquatic models: the Fish Physiology and Genomics Laboratory (INRA UR 1037-LPGP) and the Molecular Immunology and Virology Unit (INRA UR 892-VIM) and the national research infrastructure EMERG'IN".

To find out more